Our paper describing engineering of conditional mutants using CRISPR/Cas9 has been published in PLOS Genetics. The work was done in collaboration with Didier Stainier's lab at the Max Planck Institute for Heart and Lung Research. Lenny Burg (tuc33231(at)temple.edu) and I (darius(at)temple.edu) would be happy to answer any questions to help you make it work in your lab. Let's flox!
Darius
P.S. A question for linguists: would it be OK to call a Cre-reverted gene trap mutant "Crevertant"?
Darius
P.S. A question for linguists: would it be OK to call a Cre-reverted gene trap mutant "Crevertant"?
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