Publications:
Kalvaitytė M, Gabrilavičiūtė S*, Balciunas D. Rapid generation of single-insertion transgenics by Tol2 transposition in zebrafish. Dev Dyn. 2024 Jun 30. doi: 10.1002/dvdy.719. Online ahead of print. PMID: 38946125
* Vilnius University Undergraduate Student
Jiang Z, El-Brolosy MA, Serobyan V, Welker JM, Retzer N, Dooley CM, Jakutis G, Juan T, Fukuda N, Maischein HM, Balciunas D, Stainier DYR.
Parental mutations influence wild-type offspring via transcriptional adaptation. Sci Adv. 2022 Nov 25;8(47):eabj2029. doi: 10.1126/sciadv.abj2029. Epub 2022 Nov 25.
Kalvaitytė M, Balciunas D. Conditional mutagenesis strategies in zebrafish. Trends Genet. 2022 Aug;38(8):856-868. doi: 10.1016/j.tig.2022.04.007. Epub 2022 Jun 2.
Cunningham CM, Bellipanni G, Habas R, Balciunas D. Deletion of morpholino binding sites (DeMOBS) to assess specificity of morphant phenotypes. Sci Rep. 2020 Sep 21;10(1):15366. doi: 10.1038/s41598-020-71708-1.
Ichino N, Serres MR, Urban RM, Urban MD, Treichel AJ, Schaefbauer KJ, Tallant LE, Varshney GK, Skuster KJ, McNulty MS, Daby CL, Wang Y, Liao HK, El-Rass S, Ding Y, Liu W, Anderson JL, Wishman MD, Sabharwal A, Schimmenti LA, Sivasubbu S, Balciunas D, Hammerschmidt M, Farber SA, Wen XY, Xu X, McGrail M, Essner JJ, Burgess SM, Clark KJ, Ekker SC. Building the vertebrate codex using the gene breaking protein trap library. Elife. 2020 Aug 11;9:e54572. doi: 10.7554/eLife.54572.
Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, Carlson DF, Dobbs DL, McGrail M, Essner J. Efficient targeted integration directed by short homology in zebrafish and mammalian cells. Elife. 2020 May 15;9:e53968. doi: 10.7554/eLife.53968.
Tomar D, Jaña F, Dong Z, Quinn WJ 3rd, Jadiya P, Breves SL, Daw CC, Srikantan S, Shanmughapriya S, Nemani N, Carvalho E, Tripathi A, Worth AM, Zhang X, Razmpour R, Seelam A, Rhode S, Mehta AV*, Murray M, Slade D, Ramirez SH, Mishra P, Gerhard GS, Caplan J, Norton L, Sharma K, Rajan S, Balciunas D, Wijesinghe DS, Ahima RS, Baur JA, Madesh M.. Blockade of MCU-Mediated Ca2+ Uptake Perturbs Lipid Metabolism via PP4-Dependent AMPK Dephosphorylation. Cell Rep. 2019 Mar 26;26(13):3709-3725.e7.
doi: 10.1016/j.celrep.2019.02.107. * Temple University Undergraduate Student
Burg L, Palmer N*, Kikhi K, Miroshnik ES*, Rueckert H*, Gaddy E, MacPherson Cunningham C, Mattonet K, Lai SL, Marín-Juez R, Waring RB, Stainier DYR, Balciunas D. Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish. PLoS Genet. 2018 Nov 14;14(11):e1007754. doi: 10.1371/journal.pgen.1007754. * Temple University Undergraduate Student
Grajevskaja V, Camerota D*, Bellipanni G, Balciuniene J, Balciunas D. Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish. PLoS One. 2018 Jun 22;13(6):e0197293. doi: 10.1371/journal.pone.0197293. * Temple University Undergraduate Student
Balciunas D. Fish mutant, where is thy phenotype? PLoS Genet. 2018 Feb 22;14(2):e1007197. doi: 10.1371/journal.pgen.1007197.
Burg L, Zhang K*, Bonawitz T*, Grajevskaja V, Bellipanni G, Waring RW, Balciunas D. Internal epitope tagging informed by lower sequence conservation. Sci Rep. 2016 Nov 28;6:36986. doi: 10.1038/srep36986. * Temple University Undergraduate Student
Seiler C, Gebhart N, Zhang Y, Shinton SA, Li YS, Ross NL, Liu X, Li Q, Bilbee AN, Varshney GK, LaFave MC, Burgess SM, Balciuniene J, Balciunas D, Hardy RR, Kappes DJ, Wiest DL, Rhodes J. Mutagenesis Screen Identifies agtpbp1 and eps15L1 as Essential for T lymphocyte Development in Zebrafish. PLoS One. 2015 Jul 10;10(7):e0131908. doi: 10.1371/journal.pone.0131908.journals.plos.org/plosone/article?id=10.1371/journal.pone.0131908
Craig MP, Grajevskaja V, Liao HK, Balciuniene J, Ekker SC, Park JS, Essner JJ, Balciunas D, Sumanas S. Etv2 and fli1b function together as key regulators of vasculogenesis and angiogenesis. Arterioscler Thromb Vasc Biol. 2015 Apr;35(4):865-76. Epub 2015 Feb 26.
Burg L*, Gill R, Balciuniene J, Balciunas D. SideRack: a cost-effective addition to commercial zebrafish housing systems. Zebrafish. 2014;11(2):167-72. * Temple University Undergraduate Student
Roberts JA, Miguel-Escalada I, Slovik KJ*, Walsh KT*, Hadzhiev Y, Sanges R, Stupka E, Marsh EK, Balciuniene J, Balciunas D#, and Müller F#. Targeted transgene integration overcomes variability of position effects in zebrafish. Development. 2014;141(3):715-24. # co-corresponding authors * Temple University Undergraduate Student
Balciuniene J, Nagelberg D*, Walsh KT*, Camerota D*, Georlette D, Biemar F, Bellipanni G, Balciunas D. Efficient Disruption of Zebrafish Genes Using a Gal4-containing Gene Trap. BMC Genomics. 2013;4(1), 619. * Temple University Undergraduate Student
Grajevskaja V, Balciuniene J, Balciunas D. Chicken β-globin insulators fail to shield the nkx2.5 promoter from integration site effects in zebrafish. Molecular Genet. and Genomics. 2013;288(12):717-25
Balciuniene J, Balciunas D. (2013). Gene trapping using Gal4 in zebrafish. J. Vis. Exp. (79), e50113, doi:10.3791/50113
Rehn K, Wong KS, Balciunas D, Sumanas S. Zebrafish enhancer trap line recapitulates embryonic aquaporin 1a expression pattern in vascular endothelial cells. Int J Dev Biol. 2011;55(6):613-8.
Clark KJ, Balciunas D, Pogoda H-M, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J Nielsen A, Sivasubbu S, Xu X, Hammaerschmidt M, Ekker SC. In vivo protein trapping produces a functional expression codex of the vertebrate genome. Nat Methods. 2011;8(6):506-12. Epub 2011 May 8. Featured in News and Views, Nat Methods. 2011 Jun;8(6):466-7. Featured in Faculty of 1000.
Geurts A, Balciunas D, Mates L. Vertebrate Transgenesis by Transposition. In: Advanced Protocols for Animal Transgenesis: An ISTT Manual (2011). Editors: Pease S, Saunders TL. ISBN: 978-3-642-20791. Pages 213-236.
Petzold A, Balciunas D, Clark K, Spiridonov S, Sivasubbu S, Thomas MJ, Ekker SC. Nicotine Response Genetics in the Zebrafish. Proc Natl Acad Sci U S A. 2009;106(44):18662-7. Epub 2009 Oct 26.
Sivasubbu S, Balciunas D, Amsterdam A, Ekker SC. Insertional mutagenesis strategies in zebrafish. Review. Genome Biol. 2007 Oct:8(Suppl I):59.
Balciunas D, Wangensteen KJ, Wilber A, Bell J, Gurts A, Sivasubbu S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC. Harnessing a high cargo-capacity transposon for genetic applications in vertebrates. PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 2.
Balciunas D and Ekker SC. Trapping fish genes with transposons. Review. Zebrafish. 2005 1(4):335-41.
* Vilnius University Undergraduate Student
Jiang Z, El-Brolosy MA, Serobyan V, Welker JM, Retzer N, Dooley CM, Jakutis G, Juan T, Fukuda N, Maischein HM, Balciunas D, Stainier DYR.
Parental mutations influence wild-type offspring via transcriptional adaptation. Sci Adv. 2022 Nov 25;8(47):eabj2029. doi: 10.1126/sciadv.abj2029. Epub 2022 Nov 25.
Kalvaitytė M, Balciunas D. Conditional mutagenesis strategies in zebrafish. Trends Genet. 2022 Aug;38(8):856-868. doi: 10.1016/j.tig.2022.04.007. Epub 2022 Jun 2.
Cunningham CM, Bellipanni G, Habas R, Balciunas D. Deletion of morpholino binding sites (DeMOBS) to assess specificity of morphant phenotypes. Sci Rep. 2020 Sep 21;10(1):15366. doi: 10.1038/s41598-020-71708-1.
Ichino N, Serres MR, Urban RM, Urban MD, Treichel AJ, Schaefbauer KJ, Tallant LE, Varshney GK, Skuster KJ, McNulty MS, Daby CL, Wang Y, Liao HK, El-Rass S, Ding Y, Liu W, Anderson JL, Wishman MD, Sabharwal A, Schimmenti LA, Sivasubbu S, Balciunas D, Hammerschmidt M, Farber SA, Wen XY, Xu X, McGrail M, Essner JJ, Burgess SM, Clark KJ, Ekker SC. Building the vertebrate codex using the gene breaking protein trap library. Elife. 2020 Aug 11;9:e54572. doi: 10.7554/eLife.54572.
Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, Carlson DF, Dobbs DL, McGrail M, Essner J. Efficient targeted integration directed by short homology in zebrafish and mammalian cells. Elife. 2020 May 15;9:e53968. doi: 10.7554/eLife.53968.
Tomar D, Jaña F, Dong Z, Quinn WJ 3rd, Jadiya P, Breves SL, Daw CC, Srikantan S, Shanmughapriya S, Nemani N, Carvalho E, Tripathi A, Worth AM, Zhang X, Razmpour R, Seelam A, Rhode S, Mehta AV*, Murray M, Slade D, Ramirez SH, Mishra P, Gerhard GS, Caplan J, Norton L, Sharma K, Rajan S, Balciunas D, Wijesinghe DS, Ahima RS, Baur JA, Madesh M.. Blockade of MCU-Mediated Ca2+ Uptake Perturbs Lipid Metabolism via PP4-Dependent AMPK Dephosphorylation. Cell Rep. 2019 Mar 26;26(13):3709-3725.e7.
doi: 10.1016/j.celrep.2019.02.107. * Temple University Undergraduate Student
Burg L, Palmer N*, Kikhi K, Miroshnik ES*, Rueckert H*, Gaddy E, MacPherson Cunningham C, Mattonet K, Lai SL, Marín-Juez R, Waring RB, Stainier DYR, Balciunas D. Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish. PLoS Genet. 2018 Nov 14;14(11):e1007754. doi: 10.1371/journal.pgen.1007754. * Temple University Undergraduate Student
Grajevskaja V, Camerota D*, Bellipanni G, Balciuniene J, Balciunas D. Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish. PLoS One. 2018 Jun 22;13(6):e0197293. doi: 10.1371/journal.pone.0197293. * Temple University Undergraduate Student
Balciunas D. Fish mutant, where is thy phenotype? PLoS Genet. 2018 Feb 22;14(2):e1007197. doi: 10.1371/journal.pgen.1007197.
Burg L, Zhang K*, Bonawitz T*, Grajevskaja V, Bellipanni G, Waring RW, Balciunas D. Internal epitope tagging informed by lower sequence conservation. Sci Rep. 2016 Nov 28;6:36986. doi: 10.1038/srep36986. * Temple University Undergraduate Student
Seiler C, Gebhart N, Zhang Y, Shinton SA, Li YS, Ross NL, Liu X, Li Q, Bilbee AN, Varshney GK, LaFave MC, Burgess SM, Balciuniene J, Balciunas D, Hardy RR, Kappes DJ, Wiest DL, Rhodes J. Mutagenesis Screen Identifies agtpbp1 and eps15L1 as Essential for T lymphocyte Development in Zebrafish. PLoS One. 2015 Jul 10;10(7):e0131908. doi: 10.1371/journal.pone.0131908.journals.plos.org/plosone/article?id=10.1371/journal.pone.0131908
Craig MP, Grajevskaja V, Liao HK, Balciuniene J, Ekker SC, Park JS, Essner JJ, Balciunas D, Sumanas S. Etv2 and fli1b function together as key regulators of vasculogenesis and angiogenesis. Arterioscler Thromb Vasc Biol. 2015 Apr;35(4):865-76. Epub 2015 Feb 26.
Burg L*, Gill R, Balciuniene J, Balciunas D. SideRack: a cost-effective addition to commercial zebrafish housing systems. Zebrafish. 2014;11(2):167-72. * Temple University Undergraduate Student
Roberts JA, Miguel-Escalada I, Slovik KJ*, Walsh KT*, Hadzhiev Y, Sanges R, Stupka E, Marsh EK, Balciuniene J, Balciunas D#, and Müller F#. Targeted transgene integration overcomes variability of position effects in zebrafish. Development. 2014;141(3):715-24. # co-corresponding authors * Temple University Undergraduate Student
Balciuniene J, Nagelberg D*, Walsh KT*, Camerota D*, Georlette D, Biemar F, Bellipanni G, Balciunas D. Efficient Disruption of Zebrafish Genes Using a Gal4-containing Gene Trap. BMC Genomics. 2013;4(1), 619. * Temple University Undergraduate Student
Grajevskaja V, Balciuniene J, Balciunas D. Chicken β-globin insulators fail to shield the nkx2.5 promoter from integration site effects in zebrafish. Molecular Genet. and Genomics. 2013;288(12):717-25
Balciuniene J, Balciunas D. (2013). Gene trapping using Gal4 in zebrafish. J. Vis. Exp. (79), e50113, doi:10.3791/50113
Rehn K, Wong KS, Balciunas D, Sumanas S. Zebrafish enhancer trap line recapitulates embryonic aquaporin 1a expression pattern in vascular endothelial cells. Int J Dev Biol. 2011;55(6):613-8.
Clark KJ, Balciunas D, Pogoda H-M, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J Nielsen A, Sivasubbu S, Xu X, Hammaerschmidt M, Ekker SC. In vivo protein trapping produces a functional expression codex of the vertebrate genome. Nat Methods. 2011;8(6):506-12. Epub 2011 May 8. Featured in News and Views, Nat Methods. 2011 Jun;8(6):466-7. Featured in Faculty of 1000.
Geurts A, Balciunas D, Mates L. Vertebrate Transgenesis by Transposition. In: Advanced Protocols for Animal Transgenesis: An ISTT Manual (2011). Editors: Pease S, Saunders TL. ISBN: 978-3-642-20791. Pages 213-236.
Petzold A, Balciunas D, Clark K, Spiridonov S, Sivasubbu S, Thomas MJ, Ekker SC. Nicotine Response Genetics in the Zebrafish. Proc Natl Acad Sci U S A. 2009;106(44):18662-7. Epub 2009 Oct 26.
Sivasubbu S, Balciunas D, Amsterdam A, Ekker SC. Insertional mutagenesis strategies in zebrafish. Review. Genome Biol. 2007 Oct:8(Suppl I):59.
Balciunas D, Wangensteen KJ, Wilber A, Bell J, Gurts A, Sivasubbu S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC. Harnessing a high cargo-capacity transposon for genetic applications in vertebrates. PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 2.
Balciunas D and Ekker SC. Trapping fish genes with transposons. Review. Zebrafish. 2005 1(4):335-41.
