CRISPR/Cas9 genome editing methods
We have had tremendous success using CRISPR/Cas9 to introduce mutations as well as oligonucleotide-based precise edits into the zebrafish genome. We are glad to share links to our "internal" lab methods below:
Guide RNA design, synthesis and microinjection
Testing efficacy of guide RNAs
Guide RNA design, synthesis and microinjection
Testing efficacy of guide RNAs