CRISPR/Cas9 genome editing methods

We have had tremendous success using CRISPR/Cas9 to introduce mutations as well as oligonucleotide-based precise edits into the zebrafish genome. Our methodology is quite simple and robust. Screening is routinely successfully performed by undergraduate students in the lab. Click on  word or pdf links to download files describing our oligonucleotide-mediated HDR methodology.