Mutant line: tpl6         
Mutated gene: nsfa 

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-TGAGGAGCTCAACCGAGCAG-3'
Reverse: 5'-ACTTACAGGAAGATAACATG-3'
Tol2-R5: 5'-TAATCAAGTAAAATTACTCAAGTAC-3' 

Expected PCR product:
Wild type allele:  2000 bp
Gene trap allele:  350 bp

PCR notes: 
Extension temperature: 60C