Mutant line: tpl4           
Mutated gene:  stat5.1

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-CAAAAGTGGATTCAGATTAT-3'
Reverse: 5'-TCACACTAGGCCATAGTTAAGG-3'
Tol2-R5: 5'-TAATCAAGTAAAATTACTCAAGTAC-3' 

Expected PCR product:
Wild type allele: 2.2 kb
Gene trap allele: 1 kb

PCR notes: 
Annealing temperature 50C