Mutant line: tpl26      
Mutated gene:  fam89a

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-GGACATGGAGAGGATGTACGTGA-3'
Reverse: 5'-CGGAACTAATCTGTGATGCTCAGA-3'
Tol2: 5'--3' 

Expected PCR product:
Wild type allele:  1.1 kb
Gene trap allele:  kb

PCR notes: