Balciunas Lab
  • Research
    • Projects
    • Publications
    • Research Opportunities
  • People
    • Lab Life
  • News
  • Lab methods
  • Science picks
  • Publications

Mutant line: tpl25
Mutated gene:  srp68

Picture
Gene trap vector:
B1

Genotyping primers:
Forward: 5'-CAAACATGGAGACCAGCTGGAGA-3'
Reverse: 5'-CCAGTCAGATAAGCAGTGTAAGC-3'
Tol2: 5'--3' 

Expected PCR product:
Wild type allele:  1.5 kb
Gene trap allele:  kb

PCR notes: 

Powered by Create your own unique website with customizable templates.