Mutant line: tpl25
Mutated gene:  srp68

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-CAAACATGGAGACCAGCTGGAGA-3'
Reverse: 5'-CCAGTCAGATAAGCAGTGTAAGC-3'
Tol2: 5'--3' 

Expected PCR product:
Wild type allele:  1.5 kb
Gene trap allele:  kb

PCR notes: