Mutant line: tpl24
Mutated gene: cyp26c1

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-AGACGAGAGAAACATGGAAAGG-3'
Reverse: 5'-TAAGTGATTTGCTGCTCCTCC-3'
Tol2: 5'--3' 

Expected PCR product:
Wild type allele:  550 bp
Gene trap allele:  kb

PCR notes: