Mutant line: tpl23          
Mutated gene: si:ch211-225g23.2

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-GCTTACGTAATATGCAGTAGTTCA-3'
Reverse: 5'-GGGACGTTAACAGGGACATAAG-3'
Tol2: 5'--3' 

Expected PCR product:
Wild type allele:  1.8 kb
Gene trap allele:  kb

PCR notes: