Mutant line: tpl21          
Mutated gene:  triqk

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-TGCTGCGAGCCACTCGGCTGAA-3'
Reverse: 5'-GAGCTTCTCCTCTGAGAGTCAAAC-3'
Tol2: 5'--3' 

Expected PCR product:
Wild type allele:  240 bp
Gene trap allele:  kb

PCR notes: