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Mutant line: tpl20
Mutated gene:
lasp1
Gene trap vector:
B1
Genotyping primers:
Forward
: 5'-AGCTTTAGCTACTAATGTGTACC-3'
Reverse:
5'-TAGACCTGTCACGATAAGCGATT-3'
Tol2-R5
: 5'-TAATCAAGTAAAATTACTCAAGTAC-3'
Expected PCR product:
Wild type allele
: 430 bp
Gene trap allele
: 250 bp
PCR notes:
extension 60C