Mutant line: tpl18           
Mutated gene: fam46bb 

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-TGGAGACGGAGACGCCTGAGTTCAT-3'
Reverse: 5'-GTGCCAAATGGGTTAAATAAGCATG-3'
Tol2-R4: 5'-ATAATACTTAAGTACAGTAATCAAG-3' 

Expected PCR product:
Wild type allele:  600 bp
Gene trap allele:  400 bp

PCR notes: none