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Mutant line: tpl17
Mutated gene:
plecb
Gene trap vector:
B1
Genotyping primers:
Forward
: 5'-TCTATAGGAAGCAGCTTTACAATAT-3'
Reverse:
5'-GCTCATTCTGACCTCTGCCTGTCTT-3'
Tol2-F8
: 5'-CTCAAGTAAGATTCTAGCCAGATAC-3'
Expected PCR product:
Wild type allele
: 700 bp
Gene trap allele
: 400 bp
PCR notes: