Mutant line: tpl17           
Mutated gene: plecb

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-TCTATAGGAAGCAGCTTTACAATAT-3'
Reverse: 5'-GCTCATTCTGACCTCTGCCTGTCTT-3'
Tol2-F8: 5'-CTCAAGTAAGATTCTAGCCAGATAC-3' 

Expected PCR product:
Wild type allele:  700 bp
Gene trap allele:  400 bp

PCR notes: