Mutant line: tpl15      
Mutated gene:  ebf3

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-AATAGGGAGGAGGAGGAGTCTG-3'
Reverse: 5'-TGGAGGTGGCATTTAGCTTAG-3'
Tol2-R5: 5'-TAATCAAGTAAAATTACTCAAGTAC-3' 

Expected PCR product:
Wild type allele:  490 bp
Gene trap allele: 210 bp

PCR notes: