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Mutant line: tpl14
Mutated gene:
osbpl10
Gene trap vector:
B1
Genotyping primers:
Forward
: 5'-
tcaaagggatagggagatgagt
-3'
Reverse:
5'-AGCATGCAGACAAACTGCTG-3'
Tol2-R5
: 5'-TAATCAAGTAAAATTACTCAAGTAC-3'
Expected PCR product:
Wild type allele
: 700 bp
Gene trap allele
: 330 bp
PCR notes:
None