Mutant line: tpl14          
Mutated gene: osbpl10 

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-tcaaagggatagggagatgagt-3'
Reverse: 5'-AGCATGCAGACAAACTGCTG-3'
Tol2-R5: 5'-TAATCAAGTAAAATTACTCAAGTAC-3' 

Expected PCR product:
Wild type allele:  700 bp
Gene trap allele:  330 bp

PCR notes: 
None