Mutant line: tpl12          
Mutated gene: sgip1  

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-actcttctgtcagtgtgctctca-3'
Reverse: 5'-GCATATGAAGTGCGAATCCTG-3'
Tol2-R5: 5'-TAATCAAGTAAAATTACTCAAGTAC-3' 

Expected PCR product:
Wild type allele:  720 bp
Gene trap allele:  300 bp

PCR notes: