Mutant line: tpl11
Mutated gene: bbs7  

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-gcacatccatgatgcgaatc-3'
Reverse: 5'-AGCTCCTCCAAGCTCAAGTCT-3'
Tol2-R5: 5'-TAATCAAGTAAAATTACTCAAGTAC-3' 

Expected PCR product:
Wild type allele:  1 kb
Gene trap allele:  0.7 kb

PCR notes: 
Extension temperature 60C