Mutant line: tpl5           
Mutated gene: st6galnac5a

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-TGATTTGAGCATGGAAGTGTG-3'
Reverse: 5'-TGTCCACCAAGCCATCAGTA-3'
Tol2-R5: 5'-TAATCAAGTAAAATTACTCAAGTAC-3' 

Expected PCR product:
Wild type allele:   360 bp
Gene trap allele:  280 bb

PCR notes: