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Mutant line: tpl5
Mutated gene:
st6galnac5a
Gene trap vector:
B1
Genotyping primers:
Forward
: 5'-TGATTTGAGCATGGAAGTGTG-3'
Reverse:
5'-TGTCCACCAAGCCATCAGTA-3'
Tol2-R5
: 5'-TAATCAAGTAAAATTACTCAAGTAC-3'
Expected PCR product:
Wild type allele
: 360 bp
Gene trap allele
: 280 bb
PCR notes: