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Mutant line: tpl27
Mutated gene:
snap25b
Gene trap vector:
B1
Genotyping primers:
Forward
: 5'-AGAACTTTGGTTATGCTGGACG-3'
Reverse:
5'-CCTTAAATGATCCCGCCACC-3'
Tol2
: 5'--3'
Expected PCR product:
Wild type allele
: 650 bp
Gene trap allele
: kb
PCR notes: