Mutant line: tpl19
Mutated gene: flr  

Gene trap vector:
B1

Genotyping primers:
Forward: 5'-GCGTCATGTATTGATTACGTCACT-3'
Reverse: 5'-GTCTTGCATGTGATAGTAACAGTAG-3'
Gal4-R3: 5'-TAAGTCGGCAAATATCGCATGCTTG-3' 

Expected PCR product:
Wild type allele:  1.1 kb
Gene trap allele:  0.7 kb

PCR notes: